ZSCAN4 antibody (C-Term)

Catalog No. ABIN6991278

Product Details anti-ZSCAN4 Antibody

Target: ZSCAN4 (Zinc Finger and SCAN Domain Containing 4 (ZSCAN4))

Binding Specificity: AA 370-420, C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZSCAN4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Purification: ZSCAN4 Antibody is affinity chromatography purified via peptide column.

Immunogen: ZSCAN4 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human ZSCAN4. The immunogen is located within amino acids 370 - 420 of ZSCAN4.

Isotype: IgG

Application Details

Application Notes: ZSCAN4 antibody can be used for detection of ZSCAN4 by Western blot. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in mouse samples, Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: ZSCAN4 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: ZSCAN4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for ZSCAN4

Target: ZSCAN4 (Zinc Finger and SCAN Domain Containing 4 (ZSCAN4))

Alternative Name: ZSCAN4 (ZSCAN4 Products)

Synonyms: ZNF494 antibody, zinc finger and SCAN domain containing 4 antibody, ZSCAN4 antibody

Background: ZSCAN4 Antibody: Zytotic genome activation (ZGA) is the first and most critical transition event from the maternal genetic to the embryonic genetic program and the major burst of ZGA occurs only in the late 2-cell stage. ZSCAN4 encodes a SCAN domain and four zinc finger domains and is expressed in late 2-cell embryos and embryonic stem cells. Loss-of-function studies using siRNA shows delays in the progression from the 2-cell to the 4-cell stage and subsequent embryo development. ZSCAN4 plays an important role in the preimplantation development. Also, ZSCAN4 is involved in telomere maintenance and long term genomic stability in ES cells, suggesting that ZSCAN4 may be of critical importance in maintaining pluripotency in replicating stem cells.

Molecular Weight: Predicted: 48 kDa

Observed: 64 kDa

Gene ID: 201516

NCBI Accession: NP_689890

UniProt: Q8NAM6