Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Purification
MYBPC2 Antibody is affinity chromatography purified via peptide column.
Immunogen
MYBPC2 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human MYBPC2. The immunogen is located within amino acids 1070 - 1120 of MYBPC2.
MYBPC2
Reactivity: Human
WB, ELISA
Host: Rabbit
Polyclonal
unconjugated
Application Notes
MYBPC2 antibody can be used for detection of MYBPC2 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL.
Antibody validated: Western Blot in mouse samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
MYBPC2 Antibody is supplied in PBS containing 0.02 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C,4 °C
Storage Comment
MYBPC2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Target
MYBPC2
(Myosin Binding Protein C, Fast Type (MYBPC2))
MYBPC antibody, MYBPCF antibody, im:7137115 antibody, im:7150089 antibody, zgc:110761 antibody, myosin binding protein C, fast-type antibody, myosin binding protein C, fast type antibody, myosin-binding protein C, fast-type antibody, myosin binding protein C, fast type b antibody, Mybpc2 antibody, MYBPC2 antibody, LOC520988 antibody, mybpc2b antibody
Background
MYBPC2 Antibody: Myosin binding protein C (MYBPC) is a component of the thick filament of striated muscle, with the fast-type isoform designated MYBPC2. Both the fast-type and slow-type MYBPC protein contains seven immunoglobulin C2 motifs and three fibronectin type-III repeats. MYBPC2 is typically required for strong contractions and functions under anaerobic conditions. It is more similar to the cardiac isoform (MYBPC3) than to the slow-type isoform (MYBPC1) in terms of pCa50-indexed force development, length-independent cooperativity and length dependent activation. It has been suggested that in cardiac and fast muscle MYBPC2 contributes to an internal load, possibly by binding to actin via its N-terminal region.