ROCK1 antibody (N-Term)

Catalog No. ABIN6991525

This Rabbit Polyclonal antibody specifically detects ROCK1 in WB, ELISA, IF and ICC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for ROCK1 antibody (N-Term) (ABIN6991525)

Target: ROCK1 (rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ROCK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-ROCK1 Antibody

Binding Specificity: N-Term

Specificity: ROCK1 antibody is predicted to not cross-react with other ROCK protein family members.

Purification: ROCK1 Antibody is affinity chromatography purified via peptide column.

Immunogen: ROCK1 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human ROCK1. The immunogen is located within the first 50 amino acids of ROCK1.

Isotype: IgG

Application Details

Application Notes: ROCK1 antibody can be used for detection of ROCK1 by Western blot at 1 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 10 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: ROCK1 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: ROCK1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for ROCK1

Target: ROCK1 (rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1))

Alternative Name: ROCK1

Background: ROCK1 Antibody: ROCK1 is a member of the AGC serine/threonine protein kinase family that regulates the assembly of the actin cytoskeleton. ROCK1 contains a protein kinase domain, a coiled-coil region and a zinc finger region and appears to be present as a dimer. ROCK1, like its isozyme ROCK2, is a downstream target of the small G-protein Rho and plays a role in smooth muscle contraction, malignant cell transformation, tumor invasion and metastasis, and mediates the cellular morphological changes during apoptosis.

Gene ID: 6093

NCBI Accession: NP_005397

UniProt: Q13464

Pathways: Microtubule Dynamics, WNT Signaling, M Phase, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling