SPATA1 antibody (C-Term)
SPATA1
Reactivity: Human, Mouse
WB, ELISA, IF, IHC (p)
Host: Rabbit
Polyclonal
unconjugated
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- Target See all SPATA1 products
- SPATA1 (Spermatogenesis Associated 1 (SPATA1))
- Binding Specificity
- AA 300-350, C-Term
- Reactivity
- Human, Mouse
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This SPATA1 antibody is un-conjugated
- Application
- Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Specificity
- At least two isoforms of SPATA1 are known to exist, this antibody will detect only the longest isoforms. SPATA1 antibody is predicted to not cross-react with other SPATA family members.
- Purification
- SPATA1 Antibody is affinity chromatography purified via peptide column.
- Immunogen
- SPATA1 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human SPATA1. The immunogen is located within amino acids 300 - 350 of SPATA1.
- Isotype
- IgG
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- Application Notes
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SPATA1 antibody can be used for detection of SPATA1 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.
Antibody validated: Western Blot in mouse samples, Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- SPATA1 Antibody is supplied in PBS containing 0.02 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C,4 °C
- Storage Comment
- SPATA1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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- Target
- SPATA1 (Spermatogenesis Associated 1 (SPATA1))
- Alternative Name
- SPATA1 (SPATA1 Products)
- Synonyms
- SP-2 antibody, SPAP1 antibody, 4921536I21Rik antibody, AV266943 antibody, spermatogenesis associated 1 antibody, SPATA1 antibody, Spata1 antibody
- Background
- SPATA1 Antibody: SPATA1, a novel cell-surface protein, is involved in shaping the sperm head during spermatogenesis. SPATA1 is localized at chromosome 1q21. At least three isoforms of SPATA1 are known to exist. The longest is preferentially expressed in hematopoietic tissues. Its extracellular domain contains a single immunoglobulin-like domain, and its intracellular segment has two immunoreceptor tyrosine-based inhibition motifs (ITIMs). It is thought to have an important role in hematopoietic cell signaling. The mid-sized isoform contains a short intracellular part without ITIMs, while the shortest isoform lacks the transmembrane segment and represents a potential soluble protein (2, 3).
- Gene ID
- 100505741
- UniProt
- Q5VX52
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