PHLPP1 antibody (N-Term)

Catalog No. ABIN6991746

The Rabbit Polyclonal anti-PHLPP1 antibody (ABIN6991746) specifically detects PHLPP1 in WB, ELISA, IF and ICC. The antibody is reactive with Human samples.

Quick Overview for PHLPP1 antibody (N-Term) (ABIN6991746)

Target: PHLPP1 (PH Domain and Leucine Rich Repeat Protein Phosphatase 1 (PHLPP1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PHLPP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-PHLPP1 Antibody

Binding Specificity: AA 250-300, N-Term

Specificity: At least four isoforms are known to exist, this antibody will only detect the largest isoform. PHLPP1 antibody is predicted to not cross react with PHLPP2.

Purification: PHLPP1 Antibody is affinity chromatography purified via peptide column.

Immunogen: PHLPP1 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human PHLPP1. The immunogen is located within amino acids 250 - 300 of PHLPP1.

Isotype: IgG

Application Details

Application Notes: PHLPP1 antibody can be used for detection of PHLPP1 by Western blot at 1 - 2 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PHLPP1 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: PHLPP1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for PHLPP1

Target: PHLPP1 (PH Domain and Leucine Rich Repeat Protein Phosphatase 1 (PHLPP1))

Alternative Name: PHLPP1

Background: PHLPP1 Antibody: PHLPP1 is a member of the serine/threonine phosphatase family, which are important regulators of Akt serine-threonine kinases (AKT1, AKT2, AKT3) and conventional/novel protein kinase C (PKC) isoforms. PHLPP1 and PHLPP2 have a similar domain structure and have been shown to dephosphorylate and inactivate, distinct Akt isoforms, at one of the two critical phosphorylation sites required for activation: Serine473. PHLPP2 dephosphorylates AKT1 and AKT3, whereas PHLPP1 is specific for AKT2 and AKT3. PHLPP1 promotes apoptosis and may act as a tumor suppressor. Increased expression of PHLPP1 may also play a role in obesity and type 2 diabetes by interfering with Akt-mediated insulin signaling.

Molecular Weight: 189 kDa

Gene ID: 23239

NCBI Accession: NP_919431

UniProt: O60346

Pathways: PI3K-Akt Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway