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PHLPP1 antibody (N-Term)

PHLPP1 Reactivity: Human WB, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN6991746
  • Target See all PHLPP1 Antibodies
    PHLPP1 (PH Domain and Leucine Rich Repeat Protein Phosphatase 1 (PHLPP1))
    Binding Specificity
    • 5
    • 3
    • 3
    • 2
    • 1
    • 1
    AA 250-300, N-Term
    Reactivity
    • 31
    • 13
    • 12
    Human
    Host
    • 31
    Rabbit
    Clonality
    • 31
    Polyclonal
    Conjugate
    • 17
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    This PHLPP1 antibody is un-conjugated
    Application
    • 14
    • 7
    • 6
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)
    Specificity
    At least four isoforms are known to exist, this antibody will only detect the largest isoform. PHLPP1 antibody is predicted to not cross react with PHLPP2.
    Purification
    PHLPP1 Antibody is affinity chromatography purified via peptide column.
    Immunogen
    PHLPP1 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human PHLPP1. The immunogen is located within amino acids 250 - 300 of PHLPP1.
    Isotype
    IgG
    Top Product
    Discover our top product PHLPP1 Primary Antibody
  • Application Notes
    PHLPP1 antibody can be used for detection of PHLPP1 by Western blot at 1 - 2 μ,g/mL.

    Antibody validated: Western Blot in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    PHLPP1 Antibody is supplied in PBS containing 0.02 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C,4 °C
    Storage Comment
    PHLPP1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
  • Target
    PHLPP1 (PH Domain and Leucine Rich Repeat Protein Phosphatase 1 (PHLPP1))
    Alternative Name
    PHLPP1 (PHLPP1 Products)
    Synonyms
    PHLPP antibody, PLEKHE1 antibody, SCOP antibody, AI836256 antibody, Phlpp antibody, Plekhe1 antibody, mKIAA0606 antibody, Scop antibody, PH domain and leucine rich repeat protein phosphatase 1 antibody, PHLPP1 antibody, phlpp1 antibody, Phlpp1 antibody
    Background
    PHLPP1 Antibody: PHLPP1 is a member of the serine/threonine phosphatase family, which are important regulators of Akt serine-threonine kinases (AKT1, AKT2, AKT3) and conventional/novel protein kinase C (PKC) isoforms. PHLPP1 and PHLPP2 have a similar domain structure and have been shown to dephosphorylate and inactivate, distinct Akt isoforms, at one of the two critical phosphorylation sites required for activation: Serine473. PHLPP2 dephosphorylates AKT1 and AKT3, whereas PHLPP1 is specific for AKT2 and AKT3. PHLPP1 promotes apoptosis and may act as a tumor suppressor. Increased expression of PHLPP1 may also play a role in obesity and type 2 diabetes by interfering with Akt-mediated insulin signaling.
    Molecular Weight
    189 kDa
    Gene ID
    23239
    NCBI Accession
    NP_919431
    UniProt
    O60346
    Pathways
    PI3K-Akt Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway
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