PHLPP2 antibody (C-Term)

Catalog No. ABIN6991747

This Rabbit Polyclonal antibody specifically detects PHLPP2 in WB, ELISA, IF and ICC. It exhibits reactivity toward Human.

Quick Overview for PHLPP2 antibody (C-Term) (ABIN6991747)

Target: PHLPP2 (PH Domain and Leucine Rich Repeat Protein Phosphatase 2 (PHLPP2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PHLPP2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-PHLPP2 Antibody

Binding Specificity: C-Term

Specificity: At least three isoforms are known to exist, this antibody will detect the two largest isoforms. PHLPP2 antibody is predicted to not cross react with PHLPP1.

Purification: PHLPP2 Antibody is affinity chromatography purified via peptide column.

Immunogen: PHLPP2 antibody was raised against a 20 amino acid synthetic peptide near the carboxy terminus of human PHLPP2. The immunogen is located within the last 50 amino acids of PHLPP2.

Isotype: IgG

Application Details

Application Notes: PHLPP2 antibody can be used for detection of PHLPP2 by Western blot at 1 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PHLPP2 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: PHLPP2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for PHLPP2

Target: PHLPP2 (PH Domain and Leucine Rich Repeat Protein Phosphatase 2 (PHLPP2))

Alternative Name: PHLPP2

Background: PHLPP2 Antibody: PHLPP2 is a member of the serine/threonine phosphatase family, which are important regulators of Akt serine-threonine kinases (AKT1, AKT2, AKT3) and conventional/novel protein kinase C (PKC) isoforms. PHLPP1 and PHLPP2 have a similar domain structure and have been shown to dephosphorylate and inactivate, distinct Akt isoforms, at one of the two critical phosphorylation sites required for activation: Serine473. PHLPP2 dephosphorylates AKT1 and AKT3, whereas PHLPP1 is specific for AKT2 and AKT3. PHLPP1 promotes apoptosis and may act as a tumor suppressor. PHLPP2 associates with and is inhibited by adenylyl cyclase type 6 (AC6), thereby allowing Akt activation.

Molecular Weight: 156 kDa

Gene ID: 23035

NCBI Accession: NP_055835

UniProt: Q6ZVD8

Pathways: PI3K-Akt Signaling, Fc-epsilon Receptor Signaling Pathway