FLT3 antibody (AA 340-390)

Catalog No. ABIN6991750

This Rabbit Polyclonal antibody specifically detects FLT3 in WB, ELISA and IF. It exhibits reactivity toward Human and Mouse.

Quick Overview for FLT3 antibody (AA 340-390) (ABIN6991750)

Target: FLT3 (Fms-Related tyrosine Kinase 3 (FLT3))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FLT3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Product Details anti-FLT3 Antibody

Binding Specificity: AA 340-390

Specificity: At least three isoforms of FLT3 are known to exist, this antibody will detect all isoforms.

Purification: FLT3 Antibody is affinity chromatography purified via peptide column.

Immunogen: FLT3 antibody was raised against an 18 amino acid synthetic peptide near the center of human FLT3. The immunogen is located within amino acids 340 - 390 of FLT3.

Isotype: IgG

Application Details

Application Notes: FLT3 antibody can be used for detection of FLT3 by Western blot at 1 - 2 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in mouse samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: FLT3 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: FLT3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for FLT3

Target: FLT3 (Fms-Related tyrosine Kinase 3 (FLT3))

Alternative Name: FLT3

Background: FLT3 Antibody: FLT3 is a member of the class III receptor tyrosine kinase family and its internal tandem duplication (ITD) is preferentially seen in acute myeloid leukemia and myelodysplastic syndrome among various hematological malignancies. It is a membrane bound glycosylated protein that is expressed primarily in early hematopoietic progenitor cells. Upon binding its ligand FL, FLT3 activates multiple signaling pathways including the PI3K/Akt and Ras/MEK/MAPK pathways. In cells expressing FLT3-ITD, these pathways are constitutively on, resulting in abnormal cell growth and anti-apoptotic activity.

Molecular Weight: 109 kDa

Gene ID: 2322

NCBI Accession: NP_004110

UniProt: P36888

Pathways: RTK Signaling