RC3H1 antibody (C-Term)

Catalog No. ABIN6991828

This Rabbit Polyclonal antibody specifically detects RC3H1 in WB, ELISA, IHC (p) and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for RC3H1 antibody (C-Term) (ABIN6991828)

Target: RC3H1 (Ring Finger and CCCH-Type Domains 1 (RC3H1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RC3H1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Product Details anti-RC3H1 Antibody

Binding Specificity: AA 1040-1090, C-Term

Specificity: At least three isoforms of RC3H1 are known to exist, this antibody will detect all three isoforms.

Purification: RC3H1 Antibody is affinity chromatography purified via peptide column.

Immunogen: Rabbit polyclonal RC3H1 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human RC3H1. The immunogen is located within amino acids 1040 - 1090 of RC3H1.

Isotype: IgG

Application Details

Application Notes: RC3H1 antibody can be used for detection of RC3H1 by Western blot at 1 - 2 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: RC3H1 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: RC3H1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for RC3H1

Target: RC3H1 (Ring Finger and CCCH-Type Domains 1 (RC3H1))

Alternative Name: RC3H1

Background: RC3H1 Antibody: The ring finger protein RC3H1, also known as Roquin, is a highly conserved member of the RING type ubiquitin ligase protein family whose M199R mutation leads to the excessive production of follicular helper T cells and germinal centers in the sanroque strain of mice, a strain with excessive IL-21 production and high titers of autoantibodies. The complete loss of RC3H1 induces early death and immune deregulation but not autoimmunity in RC3H1-null mice, suggesting that the mutant RC3H1 is more disruptive to the immune system than its complete loss.

Molecular Weight: 125 kDa

Gene ID: 149041

NCBI Accession: NP_742068

UniProt: Q5TC82

Pathways: Ribonucleoprotein Complex Subunit Organization, Activated T Cell Proliferation