Osteopontin antibody (N-Term)

Catalog No. ABIN6991890

The Rabbit Polyclonal anti-Osteopontin antibody (ABIN6991890) specifically detects Osteopontin in WB, ELISA, IHC (p) and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for Osteopontin antibody (N-Term) (ABIN6991890)

Target: Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Osteopontin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Product Details anti-Osteopontin Antibody

Binding Specificity: AA 50-100, N-Term

Specificity: SPP1 antibody is human, mouse and rat reactive. Multiple isoforms of SPP1 are known to exist.

Purification: SPP1 Antibody is affinity chromatography purified via peptide column.

Immunogen: SPP1 antibody was raised against an 18 amino acid peptide near the amino terminus of human SPP1. The immunogen is located within amino acids 50 - 100 of SPP1.

Isotype: IgG

Application Details

Application Notes: SPP1 antibody can be used for detection of SPP1 by Western blot at 1 - 2 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: SPP1 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: SPP1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for Osteopontin

Target: Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))

Alternative Name: SPP1

Background: SPP1 Antibody: The secreted protein 1 (SPP1), also known as osteopontin, is a major noncollagenous protein of bone, but is also found in the extracellular matrix of other mineralized tissues and in bodily fluids. In bone, SPP1 is produced by osteoblasts, osteocytes, macrophages, and osteoclasts (1,2). SPP1 binds to cells through integrin and non-integrin receptors, as well as the adhesion receptor CD44 in an RGD-independent manner, enabling SPP1 to induce a number of functional effects including macrophage chemotaxis, cytoprotection, and regulation of T helper type 1 cells (2). SPP1 can regulate biomineralization by inhibiting the formation of hydroxyapatite (3) and the growth of calcium oxalate crystals (4).

Molecular Weight: Predicted: 36 kDa

Observed: 37 kDa

Gene ID: 6696

NCBI Accession: NP_001238759

UniProt: P10451

Pathways: Regulation of Cell Size