AIMP2 antibody is human specific. AIMP2 antibody is predicted to not cross-react with AIMP1.
Purification
AIMP2 antibody is affinity chromatography purified via peptide column.
Immunogen
AIMP2 antibody was raised against a 16 amino acid peptide near the center of human AIMP2. The immunogen is located within amino acids 150 - 200 of AIMP2.
AIMP2 antibody can be used for detection of AIMP2 by Western blot at 1 - 2 μ,g/mL.
Antibody validated: Western Blot in human samples, Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
AIMP2 antibody is supplied in PBS containing 0.02 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C,4 °C
Storage Comment
AIMP2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Target
AIMP2
(Aminoacyl tRNA Synthetase Complex-Interacting Multifunctional Protein 2 (AIMP2))
AIMP2 was initially identified as a part of an aminoacyl-tRNA synthesase complex (1). It was later discovered to be a cofactor and substrate of Parkin, a Ring-type E3 ubiquitin ligase that is important for the survival of dopamine neurons in Parkinson's disease, accumulation of AIMP2 in these cells lead to catecholaminergic cell death (2). AIMP2 can also bind to TRAF2, a key player in the TNF-alpha signaling pathway, causing the ubiquitination of TRAF2 by cIAP1, leading to TNF-alpha-dependent apoptosis (3). Finally, AIMP2 has been suggested to function as a tumor suppressor (4).