CASR antibody is human, mouse and rat reactive. At least two isoforms of CASR are known to exist.
Purification
CASR antibody is affinity chromatography purified via peptide column.
Immunogen
CASR antibody was raised against a 15 amino acid peptide near the amino terminus of human CASR. The immunogen is located within the first 50 amino acids of CASR.
CTNND1
Reactivity: Human, Mouse, Rat
WB, ELISA, IHC, IF
Host: Rabbit
Polyclonal
unconjugated
Application Notes
CASR antibody can be used for detection of CASR by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for Immunohistochemistry at 5 μ,g/mL. For Immunoflorescence start at 20 μ,g/mL.
Antibody validated: Western Blot in mouse samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
CASR Antibody is supplied in PBS containing 0.02 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C,4 °C
Storage Comment
CASR antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
The calcium-sensing receptor protein (CASR) is a G protein-coupled receptor that is expressed in the parathyroid hormone (PTH)-producing chief cells of the parathyroid gland, and the cells lining the kidney tubule (1). It senses small changes in circulating calcium concentration and couples this information to intracellular signaling pathways that modify PTH secretion or renal cation handling, thus this protein plays an essential role in maintaining mineral ion homeostasis (1). Mutations in this gene cause familial hypocalciuric hypercalcemia, familial, isolated hypoparathyroidism, and neonatal severe primary hyperparathyroidism (1,2). Recent evidence suggests that activated CASR contributes to the cytokine secretion through the partial MAPK and NF-kappaB pathways in T cells (3).