DEPTOR antibody (AA 220-270)

Catalog No. ABIN6992003

The Rabbit Polyclonal anti-DEPTOR antibody is suitable to detect DEPTOR in samples from Human, Mouse and Rat. It has been validated for WB, ELISA and IHC (p).

Quick Overview for DEPTOR antibody (AA 220-270) (ABIN6992003)

Target: DEPTOR (DEP Domain Containing mTOR-Interacting Protein (DEPTOR))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DEPTOR antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-DEPTOR Antibody

Binding Specificity: AA 220-270

Specificity: DEPTOR antibody is human, mouse and rat reactive. At least three isoforms of DEPTOR are known to exist, this antibody will detect all three isoforms.

Purification: DEPTOR antibody is affinity chromatography purified via peptide column.

Immunogen: DEPTOR antibody was raised against a 16 amino acid peptide near the center of human DEPTOR. The immunogen is located within amino acids 220 - 270 of DEPTOR.

Isotype: IgG

Application Details

Application Notes: DEPTOR antibody can be used for detection of DEPTOR by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for Immunohistochemistry at 5 μ,g/mL.

Antibody validated: Western Blot in mouse samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: DEPTOR Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: DEPTOR antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for DEPTOR

Target: DEPTOR (DEP Domain Containing mTOR-Interacting Protein (DEPTOR))

Alternative Name: DEPTO

Background: DEPTOR is an mTOR (mammalian target of rapamycin)-interacting protein whose expression is negatively regualted by mTOR complex 1 (mTORC1) and mTORC2 (1). Loss of DEPTOR activates RP6SK1, Akt1, and SGK1, promotes cell growth and survival, and activates mTORC1 and mTORC2 kinase activities. DEPTOR expression is low in most cancers, but is often elevated in multiple myelomas (1). In respons to stimulation by mitogens, DEPTOR is rapidly phosphorylated leading to its ubiquitination and degradation, allowing mTOR to generate an auto-amplification loop to promotes its own full activation (2).

Molecular Weight: Predicted: 45 kDa

Observed: 42 kDa

Gene ID: 64798

NCBI Accession: NP_073620

UniProt: Q8TB45

Pathways: PI3K-Akt Signaling, RTK Signaling, Regulation of Cell Size, BCR Signaling, Warburg Effect