PPARG antibody (AA 311-493)

Catalog No. ABIN7427459

This Mouse Monoclonal antibody specifically detects PPARG in WB and IHC. It exhibits reactivity toward Rat.

Quick Overview for PPARG antibody (AA 311-493) (ABIN7427459)

Target: PPARG (Peroxisome Proliferator-Activated Receptor gamma (PPARG))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This PPARG antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: C5

Product Details anti-PPARG Antibody

Binding Specificity: AA 311-493

Purpose: Monoclonal Antibody to Peroxisome Proliferator Activated Receptor Gamma (PPARg)

Specificity: The antibody is a mouse monoclonal antibody raised against PPARg. It has been selected for its ability to recognize PPARg in immunohistochemical staining and western blotting.

Purification: Protein A + Protein G affinity chromatography

Immunogen: Recombinant Peroxisome Proliferator Activated Receptor Gamma (PPARg) corresdonding to Gln311~Leu493 with N-terminal His Tag

Isotype: IgG2a kappa

Application Details

Application Notes: Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-30 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for PPARG

Target: PPARG (Peroxisome Proliferator-Activated Receptor gamma (PPARG))

Alternative Name: PPARg

Background: PPAR-G, PPARG1, PPARG2, NR1C3, Glitazone Receptor, Nuclear Receptor Subfamily 1 Group C Member 3

UniProt: O88275

Pathways: MAPK Signaling, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Regulation of Lipid Metabolism by PPARalpha, Positive Regulation of Endopeptidase Activity, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation