p53 antibody (AA 90-354)

Catalog No. ABIN7427465

This Mouse Monoclonal antibody specifically detects p53 in WB, IHC, IP and ICC. It exhibits reactivity toward Rat.

Quick Overview for p53 antibody (AA 90-354) (ABIN7427465)

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This p53 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: C2

Product Details anti-p53 Antibody

Binding Specificity: AA 90-354

Purpose: Monoclonal Antibody to Tumor Protein p53 (P53)

Specificity: The antibody is a mouse monoclonal antibody raised against P53. It has been selected for its ability to recognize P53 in immunohistochemical staining and western blotting.

Purification: Protein A + Protein G affinity chromatography

Immunogen: Recombinant Tumor Protein p53 (P53) corresdonding to Pro90~Ala354 with N-terminal His Tag

Isotype: IgG1 kappa

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for p53

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Alternative Name: Tumor Protein p53

Background: TP53, LFS1, TRP53, Li-Fraumeni Syndrome, Cellular tumor antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53

UniProt: P10361

Pathways: p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, Warburg Effect