GOT1 antibody (AA 1-413)

Catalog No. ABIN7428356

This anti-GOT1 antibody is a Mouse Monoclonal antibody detecting GOT1 in WB, IHC, IP and ICC. Suitable for Mouse.

Quick Overview for GOT1 antibody (AA 1-413) (ABIN7428356)

Target: GOT1 (Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1))

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This GOT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: C5

Product Details anti-GOT1 Antibody

Binding Specificity: AA 1-413

Purpose: Monoclonal Antibody to Aspartate Aminotransferase (AST)

Specificity: The antibody is a mouse monoclonal antibody raised against AST. It has been selected for its ability to recognize AST in immunohistochemical staining and western blotting.

Cross-Reactivity: Human, Rat

Purification: Protein A + Protein G affinity chromatography

Immunogen: Recombinant Aspartate Aminotransferase (AST) corresdonding to Met1~Gln413 with N-terminal His Tag

Isotype: IgG2b kappa

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,1:500-2000,Immunohistochemistry: 5-20 μg/mL,1:50-200,Immunocytochemistry: 5-20 μg/mL,1:50-200,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for GOT1

Target: GOT1 (Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1))

Alternative Name: Aspartate Aminotransferase

Background: CCAT, AAT, ASAT, SGOT, GOT1, Cysteine transaminase, cytoplasmic, Transaminase A, Aspartate Transaminase 1,Cytoplasmic, Glutamic-Oxaloacetic Transaminase 1,Soluble

UniProt: P05201

Pathways: Hepatitis C, Monocarboxylic Acid Catabolic Process, Methionine Biosynthetic Process