BAX antibody (AA 1-171)

Catalog No. ABIN7426265

This Mouse Monoclonal antibody specifically detects BAX in WB, IHC, IP and ICC. It exhibits reactivity toward Human.

Quick Overview for BAX antibody (AA 1-171) (ABIN7426265)

Target: BAX (BCL2-Associated X Protein (BAX))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This BAX antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: C3

Product Details anti-BAX Antibody

Binding Specificity: AA 1-171

Purpose: Monoclonal Antibody to Bcl2 Associated X Protein (Bax)

Specificity: The antibody is a mouse monoclonal antibody raised against Bax. It has been selected for its ability to recognize Bax in immunohistochemical staining and western blotting.

Cross-Reactivity: Pig

Purification: Protein A + Protein G affinity chromatography

Immunogen: Recombinant Bcl2 Associated X Protein (Bax) corresdonding to Met1~Gln171 with N-terminal His Tag

Isotype: IgG2b kappa

Application Details

Application Notes: Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.84 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: ProClin, Sodium azide

Precaution of Use: This product contains ProClin and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for BAX

Target: BAX (BCL2-Associated X Protein (BAX))

Alternative Name: Bcl2 Associated X Protein

Background: BCL2L4, Bax Zeta, Apoptosis regulator BAX, Bcl-2-like protein 4

UniProt: Q07812

Pathways: p53 Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response