GFP antibody (AA 1-238)

Catalog No. ABIN7428929

This anti-GFP antibody is a Mouse Monoclonal antibody detecting GFP in WB, IHC, IP and ICC. Suitable for Aequorea victoria.

Quick Overview for GFP antibody (AA 1-238) (ABIN7428929)

Target: GFP (Green Fluorescent Protein (GFP))

Reactivity: Aequorea victoria

Host: Mouse

Clonality: Monoclonal

Conjugate: This GFP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: C11

Product Details anti-GFP Antibody

Binding Specificity: AA 1-238

Purpose: Monoclonal Antibody to Green Fluorescent Protein (GFP)

Specificity: The antibody is a mouse monoclonal antibody raised against GFP. It has been selected for its ability to recognize GFP in immunohistochemical staining and western blotting.

Cross-Reactivity: Various Species

Purification: Protein A + Protein G affinity chromatography

Immunogen: Recombinant Green Fluorescent Protein (GFP) corresdonding to Met1~Lys238 with N-terminal His Tag

Isotype: IgG1 kappa

Application Details

Application Notes: Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for GFP

Target: GFP (Green Fluorescent Protein (GFP))

Alternative Name: Green Fluorescent Protein

UniProt: P42212