IL-1 beta antibody (AA 118-268) (APC)

Catalog No. ABIN7437425

This Guinea Pig Polyclonal antibody specifically detects IL-1 beta in WB. It exhibits reactivity toward Rabbit.

Quick Overview for IL-1 beta antibody (AA 118-268) (APC) (ABIN7437425)

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Reactivity: Rabbit

Host: Guinea Pig

Clonality: Polyclonal

Conjugate: This IL-1 beta antibody is conjugated to APC

Application: Western Blotting (WB)

Product Details anti-IL-1 beta Antibody (APC)

Binding Specificity: AA 118-268

Purpose: Polyclonal Antibody to Interleukin 1 Beta (IL1b)

Specificity: The antibody is a cavia polyclonal antibody raised against IL1b. It has been selected for its ability to recognize IL1b in immunohistochemical staining and western blotting.

Cross-Reactivity: Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Interleukin 1 Beta (IL1b) corresdonding to Val118~Ser268 (Accession # P14628)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.01-4 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for IL-1 beta

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Alternative Name: IL1b

Background: IL1-B, IL1-Beta, IL1F2, IL-1β, Interleukin-1 Family Member 2, Catabolin

UniProt: P14628

Pathways: NF-kappaB Signaling, Interferon-gamma Pathway, TLR Signaling, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy, Cancer Immune Checkpoints, Inflammasome