CD45 antibody (AA 371-556)

Catalog No. ABIN7437023

This anti-CD45 antibody is a Rabbit Polyclonal antibody detecting CD45 in IHC, WB, IP and ICC. Suitable for Mouse.

Quick Overview for CD45 antibody (AA 371-556) (ABIN7437023)

Target: CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD45 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-CD45 Antibody

Binding Specificity: AA 371-556

Purpose: Polyclonal Antibody to Protein Tyrosine Phosphatase Receptor Type C (CD45)

Specificity: The antibody is a rabbit polyclonal antibody raised against CD45. It has been selected for its ability to recognize CD45 in immunohistochemical staining and western blotting.

Cross-Reactivity: Human

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Protein Tyrosine Phosphatase Receptor Type C (CD45) corresdonding to Asp371~Asn556 (Accession # P06800) with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.01-5 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin, Sodium azide

Precaution of Use: This product contains ProClin and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for CD45

Target: CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))

Alternative Name: CD45

Background: PTPRC, PTPR-C, LCA, B220, GP180, LY5, T200, Leukocyte common antigen

UniProt: P06800

Pathways: TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, BCR Signaling