BAX antibody (AA 2-192)

Catalog No. ABIN7433498

This Rabbit Polyclonal antibody specifically detects BAX in WB, IHC, IP and ICC. It exhibits reactivity toward Pig.

Quick Overview for BAX antibody (AA 2-192) (ABIN7433498)

Target: BAX (BCL2-Associated X Protein (BAX))

Reactivity: Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BAX antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-BAX Antibody

Binding Specificity: AA 2-192

Purpose: Polyclonal Antibody to Bcl2 Associated X Protein (Bax)

Specificity: The antibody is a rabbit polyclonal antibody raised against Bax. It has been selected for its ability to recognize Bax in immunohistochemical staining and western blotting.

Cross-Reactivity: Human, Mouse, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Bcl2 Associated X Protein (Bax) corresdonding to Asp2~Gly192 (Accession # F1RIQ4)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,1:270-1100Immunohistochemistry: 5-20 μg/mL,1:27-110Immunocytochemistry: 5-20 μg/mL,1:27-110Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for BAX

Target: BAX (BCL2-Associated X Protein (BAX))

Alternative Name: Bcl2 Associated X Protein

Background: BCL2L4, Bax Zeta, Apoptosis regulator BAX, Bcl-2-like protein 4

UniProt: F1RIQ4

Pathways: p53 Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response