BRCA1 antibody (AA 1524-1781)

Catalog No. ABIN7442471

This Rabbit Polyclonal antibody specifically detects BRCA1 in WB, IHC, IP and ICC. It exhibits reactivity toward Mouse.

Quick Overview for BRCA1 antibody (AA 1524-1781) (ABIN7442471)

Target: BRCA1 (Breast Cancer 1 (BRCA1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BRCA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-BRCA1 Antibody

Binding Specificity: AA 1524-1781

Purpose: Polyclonal Antibody to Breast Cancer Susceptibility Protein 1 (BRCA1)

Specificity: The antibody is a rabbit polyclonal antibody raised against BRCA1. It has been selected for its ability to recognize BRCA1 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Breast Cancer Susceptibility Protein 1 (BRCA1) corresdonding to Phe1524~Leu1781 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for BRCA1

Target: BRCA1 (Breast Cancer 1 (BRCA1))

Alternative Name: BRCA1

Background: BRCAI, BRCC1, IRIS, PSCP, RNF53, Breast Cancer 1,Early Onset, RING finger protein 53

UniProt: P48754

Pathways: Cell Division Cycle, DNA Damage Repair, Intracellular Steroid Hormone Receptor Signaling Pathway, Positive Regulation of Response to DNA Damage Stimulus