PIAS1 antibody (AA 409-645)

Catalog No. ABIN7441808

This Rabbit Polyclonal antibody specifically detects PIAS1 in WB, IHC, ICC and IP. It exhibits reactivity toward Human.

Quick Overview for PIAS1 antibody (AA 409-645) (ABIN7441808)

Target: PIAS1 (Protein Inhibitor of Activated STAT, 1 (PIAS1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIAS1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Product Details anti-PIAS1 Antibody

Binding Specificity: AA 409-645

Purpose: Polyclonal Antibody to Protein Inhibitor Of Activated STAT 1 (PIAS1)

Specificity: The antibody is a rabbit polyclonal antibody raised against PIAS1. It has been selected for its ability to recognize PIAS1 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Protein Inhibitor Of Activated STAT 1 (PIAS1) corresdonding to Glu409~Pro645 (Accession # O75925)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for PIAS1

Target: PIAS1 (Protein Inhibitor of Activated STAT, 1 (PIAS1))

Alternative Name: PIAS1

Background: GBP, DDXBP1, GU/RH-II, ZMIZ3, Gu-binding protein, Zinc Finger,MIZ-Type Containing 3, DEAD/H(Asp-Glu-Ala-Asp/His)Box Binding Protein 1, RNA helicase II-binding protein

UniProt: O75925

Pathways: JAK-STAT Signaling, Interferon-gamma Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Muscle Cell Differentiation, Hepatitis C