HLA-E antibody (AA 22-358)

Catalog No. ABIN7434017

The Rabbit Polyclonal anti-HLA-E antibody has been validated for WB, IP, IHC and ICC. It is suitable to detect HLA-E in samples from Human.

Quick Overview for HLA-E antibody (AA 22-358) (ABIN7434017)

Target: HLA-E (HLA Class I Histocompatibility Antigen, alpha Chain E (HLA-E))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HLA-E antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-HLA-E Antibody

Binding Specificity: AA 22-358

Purpose: Polyclonal Antibody to Major Histocompatibility Complex Class I E (MHCE)

Specificity: The antibody is a rabbit polyclonal antibody raised against MHCE. It has been selected for its ability to recognize MHCE in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Major Histocompatibility Complex Class I E (MHCE) corresdonding to Gly22~Leu358 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,1:200-800,Immunohistochemistry: 5-20 μg/mL,1:20-80,Immunocytochemistry: 5-20 μg/mL,1:20-80,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin, Sodium azide

Precaution of Use: This product contains ProClin and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for HLA-E

Target: HLA-E (HLA Class I Histocompatibility Antigen, alpha Chain E (HLA-E))

Alternative Name: MHCE

Background: HLAE, EA1.2, EA2.1, HLA-6.2, MHC-E, HLA-E, Leukocyte Antigen E, MHC class I antigen E, HLA class I histocompatibility antigen, alpha chain E

UniProt: P13747

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Human Leukocyte Antigen (HLA) in Adaptive Immune Response