ERCC5 antibody (AA 778-986)

Catalog No. ABIN7434239

This Rabbit Polyclonal antibody specifically detects ERCC5 in WB, ELISA, IHC (p), ICC and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for ERCC5 antibody (AA 778-986) (ABIN7434239)

Target: ERCC5 (DNA Repair Protein Complementing XP-G Cells (ERCC5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ERCC5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-ERCC5 Antibody

Binding Specificity: AA 778-986

Purpose: Polyclonal Antibody to Xeroderma Pigmentosum, Complementation Group G (XPG)

Specificity: The antibody is a rabbit polyclonal antibody raised against XPG. It has been selected for its ability to recognize XPG in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse, Pig, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Xeroderma Pigmentosum, Complementation Group G (XPG) corresdonding to Leu778~Ala986 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 1-5 μg/mL,Immunocytochemistry in formalin fixed cells: 5-20 μg/mL,Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL,Immunohistochemistry in paraffin section: 5-20 μg/mL,Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for ERCC5

Target: ERCC5 (DNA Repair Protein Complementing XP-G Cells (ERCC5))

Alternative Name: Xeroderma Pigmentosum, Complementation Group G

Background: ERCM2, ERCC5, UVDR, XPGC, Excision Repair Cross-Complementing Rodent Repair Deficiency 5, Cockayne Syndrome, DNA repair protein complementing XP-G cells

UniProt: P28715

Pathways: DNA Damage Repair