ERCC5 antibody (AA 100-180)

Catalog No. ABIN7217516

This Rabbit Polyclonal antibody specifically detects ERCC5 in WB, ELISA, IHC and IF. It exhibits reactivity toward Human and Mouse.

Quick Overview for ERCC5 antibody (AA 100-180) (ABIN7217516)

Target: ERCC5 (DNA Repair Protein Complementing XP-G Cells (ERCC5))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ERCC5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-ERCC5 Antibody

Binding Specificity: AA 100-180

Purpose: Rabbit Anti-XPG Polyclonal Antibody

Specificity: XPG Polyclonal Antibody detects endogenous levels of XPG protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the N-terminal region of human XPG at AA range: 100-180

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:5000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for ERCC5

Target: ERCC5 (DNA Repair Protein Complementing XP-G Cells (ERCC5))

Alternative Name: XPG

Background: ERCC5, ERCM2, XPG, XPGC, DNA repair protein complementing XP-G cells, DNA excision repair protein ERCC-5, Xeroderma pigmentosum group G-complementing proteinERCC5 encodes a single-strand specific DNA endonuclease that makes the 3' incision in DNA excision repair following UV-induced damage. DNA repair protein complementing XP-G cells may also function in other cellular processes, including RNA polymerase II transcription, and transcription-coupled DNA repair. Mutations in this gene cause xeroderma pigmentosum complementation group G (XP-G), which is also referred to as xeroderma pigmentosum VII (XP7), a skin disorder characterized by hypersensitivity to UV light and increased susceptibility for skin cancer development following UV exposure. Some patients also develop Cockayne syndrome, which is characterized by severe growth defects, mental retardation, and cachexia. Read-through transcription exists between this gene and the neighboring upstream BIVM (basic, immunoglobulin-like variable motif containing) gene.

Molecular Weight: 130kD

Gene ID: 2073

UniProt: P28715

Pathways: DNA Damage Repair