SMAD6 antibody (AA 261-495)

Catalog No. ABIN7441949

The Rabbit Polyclonal anti-SMAD6 antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect SMAD6 in samples from Human.

Quick Overview for SMAD6 antibody (AA 261-495) (ABIN7441949)

Target: SMAD6 (SMAD, Mothers Against DPP Homolog 6 (SMAD6))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMAD6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-SMAD6 Antibody

Binding Specificity: AA 261-495

Purpose: Polyclonal Antibody to SMAD family member 6 (SMAD6)

Specificity: The antibody is a rabbit polyclonal antibody raised against SMAD6. It has been selected for its ability to recognize SMAD6 in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant SMAD family member 6 (SMAD6) corresdonding to Cys261~Pro495 (Accession # O43541)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,1:160-600Immunohistochemistry: 5-20 μg/mL,1:16-60Immunocytochemistry: 5-20 μg/mL,1:16-60Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.47 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for SMAD6

Target: SMAD6 (SMAD, Mothers Against DPP Homolog 6 (SMAD6))

Alternative Name: SMAD6

Background: MADH6, MADH7, Mothers Against Decapentaplegic Homolog 6

UniProt: O43541

Pathways: Chromatin Binding