MAP2K4 antibody (AA 137-394)

Catalog No. ABIN7439399

This anti-MAP2K4 antibody is a Rabbit Polyclonal antibody detecting MAP2K4 in WB, ELISA, IHC (p), IHC (fro) and ICC. Suitable for Human.

Quick Overview for MAP2K4 antibody (AA 137-394) (ABIN7439399)

Target: MAP2K4 (Mitogen-Activated Protein Kinase Kinase 4 (MAP2K4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAP2K4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)

Product Details anti-MAP2K4 Antibody

Binding Specificity: AA 137-394

Purpose: Polyclonal Antibody to Mitogen Activated Protein Kinase Kinase 4 (MAP2K4)

Specificity: The antibody is a rabbit polyclonal antibody raised against MAP2K4. It has been selected for its ability to recognize MAP2K4 in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse, Pig, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Mitogen Activated Protein Kinase Kinase 4 (MAP2K4) corresdonding to Val137~Ser394 (Accession # P45985) with N-terminal His and GST Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 1-5 μg/mL,Immunocytochemistry in formalin fixed cells: 5-20 μg/mL,Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL,Immunohistochemistry in paraffin section: 5-20 μg/mL,Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for MAP2K4

Target: MAP2K4 (Mitogen-Activated Protein Kinase Kinase 4 (MAP2K4))

Alternative Name: MAP2K4

Background: JNKK, JNKK1, MAPKK4, MEK4, MKK4, PRKMK4, SEK1, SERK1, SKK1, c-Jun N-terminal kinase kinase 1, JNK-activating kinase 1, Stress-activated protein kinase kinase 1

UniProt: P45985

Pathways: MAPK Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, BCR Signaling