SPTLC1 antibody (AA 143-473)

Catalog No. ABIN7436414

The Rabbit Polyclonal anti-SPTLC1 antibody has been validated for WB and IHC. It is suitable to detect SPTLC1 in samples from Mouse.

Quick Overview for SPTLC1 antibody (AA 143-473) (ABIN7436414)

Target: SPTLC1 (serine Palmitoyltransferase, Long Chain Base Subunit 1 (SPTLC1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPTLC1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-SPTLC1 Antibody

Binding Specificity: AA 143-473

Purpose: Polyclonal Antibody to Serine Palmitoyltransferase, Long Chain Base Subunit 1 (SPTLC1)

Specificity: The antibody is a rabbit polyclonal antibody raised against SPTLC1. It has been selected for its ability to recognize SPTLC1 in immunohistochemical staining and western blotting.

Cross-Reactivity: Human

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Serine Palmitoyltransferase, Long Chain Base Subunit 1 (SPTLC1) corresdonding to Asp143~Leu473 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes: Western blottin:g: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for SPTLC1

Target: SPTLC1 (serine Palmitoyltransferase, Long Chain Base Subunit 1 (SPTLC1))

Alternative Name: SPTLC1

Background: HSAN, HSAN1, HSN1, LBC1, LCB1, SPT1, SPTI, Hereditary Sensory Neuropathy,Type 1, Long chain base biosynthesis protein 1, Serine-palmitoyl-CoA transferase 1

UniProt: O35704