Huntingtin antibody (AA 782-920)

Catalog No. ABIN7436588

This anti-Huntingtin antibody is a Rabbit Polyclonal antibody detecting Huntingtin in IHC, WB, IP and ICC. Suitable for Mouse.

Quick Overview for Huntingtin antibody (AA 782-920) (ABIN7436588)

Target: Huntingtin (HTT)

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Huntingtin antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-Huntingtin Antibody

Binding Specificity: AA 782-920

Purpose: Polyclonal Antibody to Huntingtin (HTT)

Specificity: The antibody is a rabbit polyclonal antibody raised against HTT. It has been selected for its ability to recognize HTT in immunohistochemical staining and western blotting.

Cross-Reactivity: Human, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Huntingtin (HTT) corresdonding to Thr782~Phe920 with N-terminal His and GST Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: ProClin, Sodium azide

Precaution of Use: This product contains ProClin and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for Huntingtin

Target: Huntingtin (HTT)

Alternative Name: Huntingtin

Background: IT15, HD, Interesting Transcript 15, Huntington disease protein

UniProt: P42859

Pathways: PI3K-Akt Signaling, Hormone Transport, Transition Metal Ion Homeostasis, Tube Formation, Protein targeting to Nucleus, Dicarboxylic Acid Transport