MIB2 antibody (AA 546-784)

Catalog No. ABIN7439507

The Rabbit Polyclonal anti-MIB2 antibody has been validated for WB and IHC. It is suitable to detect MIB2 in samples from Mouse.

Quick Overview for MIB2 antibody (AA 546-784) (ABIN7439507)

Target: MIB2 (E3 ubiquitin-protein ligase MIB2 (MIB2))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MIB2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-MIB2 Antibody

Binding Specificity: AA 546-784

Purpose: Polyclonal Antibody to Mindbomb Homolog 2 (MIB2)

Specificity: The antibody is a rabbit polyclonal antibody raised against MIB2. It has been selected for its ability to recognize MIB2 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Mindbomb Homolog 2 (MIB2) corresdonding to Val546~Asn784 (Accession # Q8R516)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for MIB2

Target: MIB2 (E3 ubiquitin-protein ligase MIB2 (MIB2))

Alternative Name: MIB2

Background: ZZANK1, SKD, ZZZ5, Skeletrophin, Novelzin, Novel zinc finger protein, Zinc Finger,ZZ Type With Ankyrin Repeat Domain 1, Putative NF-kappa-B-activating protein, Mind bomb 2

UniProt: Q8R516

Pathways: Notch Signaling