TIGIT antibody (AA 28-227)

Catalog No. ABIN7442084

The Rabbit Polyclonal anti-TIGIT antibody (ABIN7442084) specifically detects TIGIT in WB, IHC, ICC and IP. The antibody is reactive with Rat samples.

Quick Overview for TIGIT antibody (AA 28-227) (ABIN7442084)

Target: TIGIT (T Cell Immunoreceptor with Ig and ITIM Domains (TIGIT))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TIGIT antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Product Details anti-TIGIT Antibody

Binding Specificity: AA 28-227

Purpose: Polyclonal Antibody to T-Cell Immunoreceptor With Ig And ITIM Domains Protein (TIGIT)

Specificity: The antibody is a rabbit polyclonal antibody raised against TIGIT. It has been selected for its ability to recognize TIGIT in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant T-Cell Immunoreceptor With Ig And ITIM Domains Protein (TIGIT) corresdonding to Met28~Ser227 (Accession # D3ZTQ2)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for TIGIT

Target: TIGIT (T Cell Immunoreceptor with Ig and ITIM Domains (TIGIT))

Alternative Name: TIGIT

Background: VSIG9, VSTM3, V-Set And Immunoglobulin Domain Containing 9, V-Set And Transmembrane Domain Containing 3

UniProt: D3ZTQ2

Pathways: Cancer Immune Checkpoints