HMGB4 antibody

Catalog No. ABIN7239995

Product Details anti-HMGB4 Antibody

Target: HMGB4 (High Mobility Group Box 4 (HMGB4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HMGB4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Characteristics: Polyclonal Antibody

Purification: Affinity purification

Immunogen: Recombinant protein of human HMGB4

Isotype: IgG

Application Details

Application Notes: WB 1:200-1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.05 % sodium azide and 50 % glycerol, PH7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for HMGB4

Target: HMGB4 (High Mobility Group Box 4 (HMGB4))

Alternative Name: HMGB4 (HMGB4 Products)

Synonyms: HMGB4 antibody, dJ1007G16.5 antibody, 1700001F22Rik antibody, AI326135 antibody, high mobility group box 4 antibody, high-mobility group box 4 antibody, HMGB4 antibody, Hmgb4 antibody

Background: High mobility group protein B4is atranscription factorthat in humans is encoded by theHMGB4gene.HMGB4 is strongly and preferentially expressed in the adult mouse testis and weakly in the brain, but not in many other tissues. HMGB4 associates with chromatin, and in transfection assays, in contrast to HMGB1, it acts as a potent transcriptional repressor. During spermatogenesis, HMGB4 is present in the euchromatin of late pachytene spermatocytes and haploid round spermatids, whereas stronger expression is observed during the elongation phase, where it localizes to the basal pole of the nucleus in a manner mutually exclusive with H1FNT (H1T2) localized at the apical pole. HMGB4 basal localization is lost in H1FNT-mutant spermatids, showing that H1FNT provides a positional cue for organizing chromatin domains within the nucleus. These results show that HMGB4 and H1FNT specify distinct chromatin domains at the apical and basal poles of the elongating spermatid nucleus.

Molecular Weight: 22 kDa

UniProt: Q8WW32