RPS6KB1 antibody (pSer418)

Catalog No. ABIN7251001

This Rabbit Polyclonal antibody specifically detects RPS6KB1 in WB, ELISA, IF and IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for RPS6KB1 antibody (pSer418) (ABIN7251001)

Target: RPS6KB1 (Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RPS6KB1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-RPS6KB1 Antibody

Binding Specificity: pSer418

Characteristics: Phosphorylated antibody

Purification: Affinity purification

Immunogen: Synthesized peptide derived from human p70 S6 kinase alpha around the phosphorylation site of Ser418

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:100-1:300, IF 1:200-1:1000, ELISA 1:20000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % sodium azide, 0.5 % BSA and 50 % glycerol, pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for RPS6KB1

Target: RPS6KB1 (Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1))

Alternative Name: p70 S6 kinase alpha

Background: P70 S6 Kinase alpha is a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases and contains 2 non-identical kinase catalytic domains. It phosphorylates several residues of the S6 ribosomal protein. The kinase activity of this protein is activated by serine/threonine phosphorylation and protein kinase C and inactivated by type 2A phosphatase. Activation leads to an increase in protein synthesis and cell proliferation. Overexpression of this kinase is involved in some breast cancer cell lines. Alternate translational start sites have been described and alternate transcriptional splice variants have been observed but have not been thoroughly characterized.

Molecular Weight:

Observed_MW: 60 kDa

Calculated_MW: 59 kDa

UniProt: P23443

Pathways: PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Regulation of Cell Size, Skeletal Muscle Fiber Development, Feeding Behaviour, G-protein mediated Events, Smooth Muscle Cell Migration, Interaction of EGFR with phospholipase C-gamma, Warburg Effect