Tuberin antibody (AA 1601-1750)

Catalog No. ABIN701200

This Rabbit Polyclonal antibody specifically detects Tuberin in ELISA, IF (cc), IF (p), IHC (p) and IHC (fro). It exhibits reactivity toward Human, Mouse and Ratand has been mentioned in 1 publication.

Quick Overview for Tuberin antibody (AA 1601-1750) (ABIN701200)

Key Features:

• High quality Tuberin Primary Antibody for the detection of TSC2.
• Reliable product with high standard validation data

Target: Tuberin (TSC2) (Tuberous Sclerosis 2 (TSC2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Tuberin antibody is un-conjugated

Application: ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Tuberin Antibody

Binding Specificity: AA 1601-1750

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Dog,Cow,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Tuberin

Isotype: IgG

Product Specific Information:

What can the Tuberin Antibody ABIN701200 be used for?: This polyclonal Tuberin Antibody detects Tuberin. The Tuberin Antibody has been validated for various applications and can be used for the detection of Tuberin and derivatives by ELISA, Immunofluorescence (Cultured Cells), Immunofluorescence (Paraffin-embedded Sections), Immunohistochemistry (Paraffin-embedded Sections), Immunohistochemistry (Frozen Sections).

What validation data is available for this Tuberin Antibody?: The primary antibody is referenced in 1 publications and is characterized by a proven, very high reliability. It has currently 3 product images that show its performance in a variety of applications. The product is currently available in 100 μL quantity. Tuberin Antibody for the detection of Tuberin and derivatives.

What is the function of Tuberin?: Mutations in this gene lead to tuberous sclerosis complex. Its gene product is believed to be a tumor suppressor and is able to stimulate specific GTPases. The protein associates with hamartin in a cytosolic complex, possibly acting as a chaperone for hamartin. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008].

Application Details

Application Notes: ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-Tuberin antibody (ABIN701200)

Wang, Chen, Deng, Bian, Huang, Lei, Lv, Liu, Li: "Repression of TSC1/TSC2 mediated by MeCP2 regulates human embryo lung fibroblast cell differentiation and proliferation." in: International journal of biological macromolecules, Vol. 96, pp. 578-588, (2017) (PubMed).

Target Details for Tuberin

Target: Tuberin (TSC2) (Tuberous Sclerosis 2 (TSC2))

Alternative Name: Tuberin

Background:

Synonyms: LAM, TSC4, PPP1R160, Tuberin, Tuberous sclerosis 2 protein, TSC2

Background: In complex with TSC1, inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. Acts as a GTPase-activating protein (GAP) for the small GTPase RHEB, a direct activator of the protein kinase activity of mTORC1. Implicated as a tumor suppressor. Involved in microtubule-mediated protein transport, but this seems to be due to unregulated mTOR signaling. Stimulates weakly the intrinsic GTPase activity of the Ras-related proteins RAP1A and RAB5 in vitro. Mutations in TSC2 lead to constitutive activation of RAP1A in tumors.

Gene ID: 7249

UniProt: P49815

Pathways: RTK Signaling, AMPK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Cell Size, Tube Formation, Protein targeting to Nucleus