GLI1 antibody (Internal Region)

Catalog No. ABIN7013859

Product Details anti-GLI1 Antibody

Target: GLI1 (Zinc Finger Protein GLI1 (GLI1))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This GLI1 antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Purpose: GLI1

Sequence: TYSQCPRLEH YGQ

Specificity: This antibody is expected to recognize the reported isoforms (NP_005260.1, NP_001153517.1, NP_001161081.1).

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for GLI1

Target: GLI1 (Zinc Finger Protein GLI1 (GLI1))

Alternative Name: GLI1 (GLI1 Products)

Synonyms: GLI antibody, Gli antibody, Gli-1 antibody, gli1.1 antibody, AV235269 antibody, Zfp-5 antibody, Zfp5 antibody, GLI family zinc finger 1 antibody, GLI family zinc finger 1 S homeolog antibody, GLI-Kruppel family member GLI1 antibody, GLI1 antibody, Gli1 antibody, gli1.S antibody, gli1 antibody

Background: GLI1, GLI family zinc finger 1, GLI, glioma-associated oncogene family zinc finger 1, glioma-associated oncogene homolog 1 (zinc finger protein), oncogene GLI, zinc finger protein GLI1

Gene ID: 2735, 14632, 140589

Pathways: Hedgehog Signaling, Dopaminergic Neurogenesis