TLR4 antibody (Internal Region)

Catalog No. ABIN7013877

This Goat Polyclonal antibody specifically detects TLR4 in FACS, IHC, ELISA and IF. It exhibits reactivity toward Human and Mouse.

Quick Overview for TLR4 antibody (Internal Region) (ABIN7013877)

Target: TLR4 (Toll-Like Receptor 4 (TLR4))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This TLR4 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Grade: Verified

Product Details anti-TLR4 Antibody

Binding Specificity: Internal Region

Purpose: TLR4 / CD284

Sequence: YSSQDEDWVR NE

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Spleen. Recommended concentration: 5-6 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:16000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of NIH3T3 cells. Recommended concentration: 10μg/ml

Flow Cytometry: Flow cytometric analysis of U937 cells. Recommended concentration: 10ug/ml

Restrictions: For Research Use only

Handling

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for TLR4

Target: TLR4 (Toll-Like Receptor 4 (TLR4))

Alternative Name: TLR4

Background: TLR4, toll-like receptor 4, ARMD10, CD284, TOLL, hToll, OTTHUMP00000022807, homolog of Drosophila toll

Gene ID: 7099, 21898, 29260

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades, Inflammasome, S100 Proteins