ADCY3 antibody (Extracellular)

Catalog No. ABIN7042892

The Rabbit Polyclonal anti-ADCY3 antibody has been validated for WB, IHC, IF, FACS, IC and LCI. It is suitable to detect ADCY3 in samples from Rat.

Quick Overview for ADCY3 antibody (Extracellular) (ABIN7042892)

Target: ADCY3 (Adenylate Cyclase 3 (ADCY3))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ADCY3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunochromatography (IC), Live Cell Imaging (LCI)

Product Details anti-ADCY3 Antibody

Binding Specificity: AA 285-299, Extracellular

Purpose: A Rabbit Polyclonal Antibody to Adenylate Cyclase Type III

Specificity: 3rd extracellular loop

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,human - identical

Characteristics: Anti-Adenylate Cyclase 3 (AC3) (extracellular) Antibody (ABIN7042892, ABIN7043932 and ABIN7043933) is a highly specific antibody directed against an extracellular epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry and live cell imaging applications. The antibody recognizes an extracellular epitope and thus is ideal for detecting ADCY3 in living cells. It has been designed to recognize ADCY3 from human, rat and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KHVADEMLKDMKKDE, corresponding to amino acid residues 285-299 of rat ADCY3

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:400

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: LCI

Negative Control: (ABIN7234646)

Blocking Peptide: (ABIN7234646)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for ADCY3

Target: ADCY3 (Adenylate Cyclase 3 (ADCY3))

Alternative Name: ADCY3

Background: Adenylate cyclase type 3, Adenylate cyclase type III, AC-III, ATP pyrophosphate-lyase 3, Adenylate cyclase olfactive type, Adenylyl cyclase 3, ADCY3,Adenylyl cyclases (ACs) are a family of enzymes that synthesize cAMP, upon stimulation. cAMP is an important second messenger, which regulates carbohydrate, lipid, protein, and nucleic acid metabolism as well as synaptic transmission, ion channel function, and transcription in neurons1. There are nine closely related isoforms of ACs (AC1-9) in mammals. All AC isoforms share a primary structure consisting of two hydrophobic regions which comprise six transmembrane helices and three large cytoplasmic domains. The basic catalytic unit of a membrane-bound AC molecule consists of the C1a and C2 domains. The N-terminus domains of ACs are highly variable, and might play a regulatory role2.Adenylyl cyclase type 3 (AC3) is widely expressed in the olfactory tissue, human cell line HEK-293, bovine brain, spinal cord, adrenal medulla, adrenal cortex, heart atrium, aorta, lung and retina3.Several studies have suggested that AC3 may play an important role in the regulation of glucose metabolism and body weight. It was found that overexpression of AC3 mRNA is involved in pathophysiological metabolic processes, such as type 2 diabetes4. In addition, a recent study demonstrated the association of AC3 with gastric cancer as well as its tumorigenic potentials5.Furthermore, AC3-knockout mice showed peripheral and behavioral anosmia6.

Alternative names: Adenylate Cyclase 3 (AC3), Adenylate cyclase type 3, Adenylate cyclase type III, AC-III, ATP pyrophosphate-lyase 3, Adenylate cyclase olfactive type, Adenylyl cyclase 3, ADCY3

Gene ID: 64508

NCBI Accession: NM_001320613

UniProt: P21932

Pathways: EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma