ADRB1 antibody (2nd Extracellular Loop) (Atto 488)

Catalog No. ABIN7042903

Product Details anti-ADRB1 Antibody (Atto 488)

Target: ADRB1 (Adrenergic, beta-1-, Receptor (ADRB1))

Binding Specificity: 2nd Extracellular Loop, AA 200-214

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ADRB1 antibody is conjugated to Atto 488

Application: Immunohistochemistry (IHC), Immunofluorescence (IF)

Characteristics: Anti-β1-Adrenergic Receptor (extracellular) Antibody (ABIN7042902, ABIN7043924 and ABIN7043925)) is a highly specific antibody directed against an extracellular epitope the rat β1-adrenoceptor. The antibody can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting the receptor in living cells. It has been designed to recognize β1AR from mouse, rat and human samples. \nAnti-β1-Adrenergic Receptor (extracellular)-ATTO Fluor-488 Antibody (#ABIN7042903) is directly labeled with an ATTO-488 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-488 label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. Anti-β1-Adrenergic Receptor (extracellular)-ATTO Fluor-488 Antibody has been tested in immunohistochemistry applications and is especially suited for experiments requiring simultaneous labeling of different markers.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)RAESDEARR(S)YNDP, corresponding to amino acid residues 200-214 of rat ADRB1 

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 50 μL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1 % BSA, 0.05 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for ADRB1

Target: ADRB1 (Adrenergic, beta-1-, Receptor (ADRB1))

Alternative Name: beta1-Adrenergic Receptor (ADRB1 Products)

Synonyms: ADRB1R antibody, B1AR antibody, BETA1AR antibody, RHR antibody, RATB1AR antibody, X-Beta1AR antibody, BAR1 antibody, zgc:194728 antibody, zgc:194731 antibody, ADRB1 antibody, Adrb-1 antibody, beta-AR antibody, adrenoceptor beta 1 antibody, adrenoceptor beta 1 L homeolog antibody, adrenergic, beta-1-, receptor antibody, adrenergic receptor, beta 1 antibody, ADRB1 antibody, Adrb1 antibody, adrb1.L antibody, adrb1 antibody

Background: Alternative names: beta1-Adrenergic Receptor, Beta-1 adrenoceptor, Beta-1 adrenoreceptor, ADRB1, ADRB1R, B1AR

Gene ID: 24925

NCBI Accession: NM_000684

UniProt: P18090

Pathways: cAMP Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Synaptic Membrane, Regulation of G-Protein Coupled Receptor Protein Signaling, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma, Brown Fat Cell Differentiation