BDNF antibody (Intracellular)

Catalog No. ABIN7042970

The Rabbit Polyclonal anti-BDNF antibody has been validated for WB, IHC, IF and IC. It is suitable to detect BDNF in samples from Human.

Quick Overview for BDNF antibody (Intracellular) (ABIN7042970)

Target: BDNF (Brain-Derived Neurotrophic Factor (BDNF))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BDNF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC)

Grade: KO Validated

Product Details anti-BDNF Antibody

Binding Specificity: AA 166-178, Intracellular

Purpose: A Rabbit Polyclonal Antibody to BDNF

Specificity: Intracellular

Cross-Reactivity: Human, Mouse, Rat

Cross-Reactivity (Details): The antibody is specific for BDNF, it does not crossreact with NGF, NT-3 or NT-4.

Predicted Reactivity: Mouse,rat,many other species - identical

Characteristics: Anti-BDNF Antibody (ABIN7042970, ABIN7044761 and ABIN7044762) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize BDNF from rat, human, and mouse samples. The antibody is specific for BDNF, it does not crossreact with NGF, or .

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)VLEKVPVSKQLK, corresponding to amino acid residues 166-178 of human BDNF (precursor)

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300

Application Dilutions Western blot wb: 1:200

Comment:

Positive Control: PCP-B250

Cited Application: ICC

Negative Control: (ABIN7234876)

Blocking Peptide: (ABIN7234876)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for BDNF

Target: BDNF (Brain-Derived Neurotrophic Factor (BDNF))

Alternative Name: BDNF

Background: Brain-derived neurotrophic factor,Brain-derived neurotrophic factor (BDNF) is a member of the neurotrophin family of growth factors which includes nerve growth factor (NGF), neurotrophin-3 (NT-3) and neurotrophin-4/5 (NT-4/5).All neurotrophins are synthesized as preproneurotrophin precursors that are subsequently processed within the intracellular transport pathway to yield proneurotrophins that are further processed to generate the mature form. The mature form of BDNF is a non-covalent stable homodimer that can be secreted in both constitutive and regulated pathways.BDNF conveys its activity by binding to two classes of receptors, a member of the Trk receptor tyrosine kinase family (TrkB) and the pan-neurotrophin receptor p75NTR. Binding of BDNF to the TrkB receptor triggers ligand-induced dimerization and autophosphorylation of tyrosine residues. This activates various signaling cascades such the MAPK, PI3K and PLCγ pathways that are involved in cell growth, survival and differentiation of neurons in the central and peripheral nervous system.Interestingly, recent evidence suggests that BDNF may influence target cell function via ion channel modulation. Ion channel activity in the target cells can be modulated by a TrkB-mediated mechanism that has not yet been determined. BDNF is able to block both KV1.3 and AMPA-subtype glutamate ion channel currents in sensory neurons, while it can induce activation of the TRPC3 cation channel in neurons and of the NaV1.9 Na+ channel in hippocampal neurons. These newly recognized BDNF actions underlie its "rapid" neuronal functions that include changes in neuronal excitability, plasticity and synaptic transmission.

Alternative names: Brain Derived Neurotrophic Factor, Brain Derived Neurotrophic Factor

Gene ID: 627

NCBI Accession: NM_170735

UniProt: P23560

Pathways: RTK Signaling, Synaptic Membrane, Feeding Behaviour, Dicarboxylic Acid Transport, Regulation of long-term Neuronal Synaptic Plasticity