Calcitonin Receptor antibody (1st Extracellular Loop)

Catalog No. ABIN7042978

This Rabbit Polyclonal antibody specifically detects Calcitonin Receptor in WB, IF, IC and LCI. It exhibits reactivity toward Rat.

Quick Overview for Calcitonin Receptor antibody (1st Extracellular Loop) (ABIN7042978)

Target: Calcitonin Receptor (CALCR)

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Calcitonin Receptor antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunochromatography (IC), Live Cell Imaging (LCI)

Product Details anti-Calcitonin Receptor Antibody

Binding Specificity: 1st Extracellular Loop, AA 201-215

Purpose: A Rabbit Polyclonal Antibody to Calcitonin Receptor

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Mouse - identical, human - 14,15 amino acid residues identical

Characteristics: Anti-Calcitonin Receptor (extracellular) Antibody is directed against an epitope of rat CT-R. Anti-Calcitonin Receptor Antibody (ABIN7042978, ABIN7044190 and ABIN7044191) can be used in western blot and live cell imaging applications. It has been designed to recognize CT-R from human, rat and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)HLVEVVPNGDLVRRD, corresponding to amino acid residues 201-215 of rat CALCR

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: 1:50

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7234900)

Blocking Peptide: (ABIN7234900)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for Calcitonin Receptor

Target: Calcitonin Receptor (CALCR)

Alternative Name: CALCR

Background:

Synonyms: CALCR, CT receptor, CT-R, C1A/C1B

Description: the calcitonin receptor (CTR) is a class B G-protein coupled receptor (GPCR) that signals via activation of cAMP and protein kinase C (PKC). It is one of 15 human class B GPCRs1,2. Apart from CTR, calcitonin receptor-like receptor (CTLR) is the only other B-class GPCR to bind calcitonin. To date, there are three known human CTR mRNA isoforms arising from alternative splicing3.Calcitonin itself is a peptide hormone that contributes to bone calcification by direct inhibition of osteoclasts which resorb the bony tissue. It is part of a six-member family that has varied functionalities. By binding to receptor activity modifying proteins (RAMPs), CTR and CTLR can form receptors to every member of the calcitonin family. For instance, RAMP1 and CTR construct the calcitonin gene-related peptide (CGRP) receptor.Both CTR and CTLR have seven transmembrane domains, an intracellular C-terminal, and an extracellular N-terminal. To activate the receptors, their N-terminal must first capture the C-terminal of the ligand and then carried to the transmembrane loops where it bestows a conformational change upon them.Besides the bone, CTR has prominent roles in the kidney, CNS, placenta, ovaries, sperm, lung, and in the brain4,5.In osteoclasts, the expression of CTRs declines with age. Interestingly, polymorphism of the CTR gene is strongly associated with decreased fracture-risk in post-menopausal women3,6.

Gene ID: 116506

NCBI Accession: NM_001742

UniProt: P32214

Pathways: cAMP Metabolic Process