CLIC5 antibody (Intracellular)

Catalog No. ABIN7043058

Product Details anti-CLIC5 Antibody

Target: CLIC5 (Chloride Intracellular Channel 5 (CLIC5))

Binding Specificity: AA 161-174, Intracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CLIC5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC)

Purpose: A Rabbit Polyclonal Antibody to CLIC5 Channel

Specificity: Intracellular, C-terminus

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Mouse - identical, human - 9,14 amino acid residues identical

Characteristics: Anti-CLIC5 Antibody (ABIN7043058, ABIN7044141 and ABIN7044142) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunocytochemistry and immunohistochemistry applications. It has been designed to recognize CLIC5 from mouse, rat, and human samples.

Purification: Affinity purified on immobilized antigen.

Grade: KO Validated

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)DTNTHGDEKGSQRK, corresponding to amino acid residues 161-174 of rat CLIC5

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: (ABIN7235110)

Blocking Peptide: (ABIN7235110)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for CLIC5

Target: CLIC5 (Chloride Intracellular Channel 5 (CLIC5))

Alternative Name: CLIC5 (CLIC5 Products)

Background: Cl- intracellular channel 5,Chloride (Cl-) channels are membrane, anion-selective protein pores, which diffusively transport negative ions across their electrochemical gradient. Cl- channels are subdivided into six unique families, and their regulation mechanisms range from voltage-dependency, through G-protein activation, to mechanosensitivity, they may reside in either the plasma membrane or the membrane of intracellular compartments or both1. They are detected in the kidney, placenta, intestines, brain and the inner-ear1-4. The vertebrate Cl- intracellular channel (CLIC) family is composed of 6 highly conserved members (CLIC1-6), each exists as a ~250 residue protein which can assume both a soluble fold and a membrane-integral form. The latter consists of a single trans-membrane domain and is subsequently oligomerized to construct a functioning channel1,5. Interestingly, channels composed of CLIC4 and CLIC5 have been confirmed to be equally permeable to K+ and Cl-5.CLIC5, to which there are two isoforms - CLIC5A and CLIC5B - encoded by the same gene5, has first been isolated from human placenta6, and was shown, together with CLIC4 (with which it shares 76 % homology), to be enriched in the apical microvilli-containing part of the trophoblast epithelium, where, unlike CLIC4, it interacts with the actin cytoskeleton4. CLIC5 shares about 40 % homology with CLIC1-4 and is distributed to a high degree in myocytes, cardiomyocytes and stereocillia of the inner-ear2,4. Mice mutated in the Clic5 gene lack coordination and gradually become deaf2, show resistance to obesity, experience gastric ulcers and the resultant hemorrhage, and have higher incidence of entering torpor7.

Alternative names: CLIC5, Cl-  intracellular channel 5

Gene ID: 94272

NCBI Accession: NM_001114086

UniProt: Q9EPT8

Pathways: Sensory Perception of Sound