GRIK2 antibody (Intracellular)

Catalog No. ABIN7043238

Product Details anti-GRIK2 Antibody

Target: GRIK2 (Glutamate Receptor, Ionotropic, Kainate 2 (GRIK2))

Binding Specificity: Intracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRIK2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunochromatography (IC)

Purpose: A Rabbit Polyclonal Antibody to Kainate Receptor GluK2

Specificity: Intracellular, C-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Human,mouse,dog - identical

Characteristics: Anti-GRIK2 (GluK2) Antibody (ABIN7043238, ABIN7044336 and ABIN7044337) is directed against the intracellular C-terminus domain of the rat kainate receptor GluK2. The antibody can be used in western blot and immunofluorescence applications and will recognize GluR6 from rat, human and mouse origins.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: CSAMVEELRMSLK, corresponding to amino acid residues 858-870 of rat kainate receptor GluK2

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:400

Comment:

Negative Control: (ABIN7235657)

Blocking Peptide: (ABIN7235657)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for GRIK2

Target: GRIK2 (Glutamate Receptor, Ionotropic, Kainate 2 (GRIK2))

Alternative Name: GRIK2 (GRIK2 Products)

Background: Glutamate receptor ionotropic kainate 2, Glutamate receptor 6, GluR6, GLUK6,L-Glutamate, the major excitatory neurotransmitter in the central nervous system (CNS), operates through several receptors that are categorized as ionotropic (ligand-gated cation channels) or metabotropic (G-protein-coupled receptors).The ligand-gated ion channel family consists of fifteen members that have been subdivided into three families based upon their pharmacological profile: the α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)-preferring receptors, the N-methyl-D-aspartate (NMDA)-preferring receptors, and the kainate-preferring receptors.The kainate receptor subfamily consists of five members that have been further subdivided into two classes based upon structural homology and functional characteristics. GluR5, GluR6, and GluR7 receptor subunits share a high degree of homology and are able to form functional channels when expressed in heterologous systems. The KA-1 and KA-2 receptors are unable to form functional channels on their own, but when coexpressed with GluR5-7 receptor subunits, they form channels with high affinity for kainate.1,2Like AMPA receptors, the functional unit of endogenous kainate receptors is believed to be a tetramer, which can be either homomeric or heteromeric. Kainate receptors GluR5 and GluR6 (but not GluR7, KA-1, or KA-2) can undergo RNA editing, as in the AMPA receptor GluR2, a glutamine (Q) residue in the channel pore is edited to encode arginine (R) in the mature protein. Substitution of Q with R modulates the properties of the channel, producing channels with reduced single channel conductance and lower permeability to Ca2+.1,2GluR6 is expressed in the CNS in basal ganglia, cerebellum, and hipoccampus, as well as in the spinal cord. The exact physiological role of GluR6 is unclear, but a role in controlling neuronal excitability, synaptic integration, and synaptic plasticity has been proposed.

Alternative names: GRIK2 (GluK2), Glutamate receptor ionotropic kainate 2, Glutamate receptor 6, GluR6, GLUK6

Gene ID: 54257

NCBI Accession: NM_001166247

UniProt: P42260

Pathways: Synaptic Membrane, Regulation of long-term Neuronal Synaptic Plasticity