Metabotropic Glutamate Receptor 5 antibody (Extracellular)

Catalog No. ABIN7043250

Product Details anti-Metabotropic Glutamate Receptor 5 Antibody

Target: Metabotropic Glutamate Receptor 5 (GRM5) (Glutamate Receptor, Metabotropic 5 (GRM5))

Binding Specificity: AA 367-380, Extracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Metabotropic Glutamate Receptor 5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunofluorescence (Cultured Cells) (IF (cc)), Immunochromatography (IC), Live Cell Imaging (LCI)

Purpose: A Rabbit Polyclonal Antibody to Metabotropic Glutamate Receptor 5

Specificity: Extracellular, N-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse - identical, human - 13,14 amino residues identical

Characteristics: Anti-mGluR5 (extracellular) Antibody (ABIN7043250, ABIN7044332 and ABIN7044333)) is a highly specific antibody directed against an extracelluar epitope of rat mGluR5. The antibody can be used in western blot, immunohistochemistry, live cell imaging, and indirect live cell flow cytometry applications. It has been designed to recognize mGluR5 from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: EGFAQENSKYNKTC, corresponding to amino acids 367-380 of rat mGluR5

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:50

Application Dilutions Western blot wb: 1:500

Comment:

Negative Control: BLP-GC007

Blocking Peptide: BLP-GC007

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for Metabotropic Glutamate Receptor 5

Target: Metabotropic Glutamate Receptor 5 (GRM5) (Glutamate Receptor, Metabotropic 5 (GRM5))

Alternative Name: GRM5 (GRM5 Products)

Background: Metabotropic glutamate receptor 5, GRM5, mGlu5, GPRC1E,L-Glutamate, the major excitatory neurotransmitter in the central nervous system, operates through several receptors that are categorized as ionotropic (ligand-gated cation channels) or metabotropic (G-protein coupled receptors). The metabotropic glutamate receptors family includes eight members (mGluR1-8) that have been divided into three groups based on their sequence homology, pharmacology and signal transduction.Group I of the metabotropic glutamate receptors includes the mGluR1 and mGluR5 receptors. The receptors present the typical G-protein coupled receptor (GPCR) signature topology: seven transmembrane domains with a large extracellular N-terminus domain and an intracellular C-terminus one. The N-terminus domain of group I receptors contains the glutamate binding site while the cytoplasmic C-terminus domain has an important role in the regulation of receptor activity through interactions with other proteins such as the Homer adaptor proteins.mGluR1 and mGluR5 receptors signal through Gq/G11 that activates phospholipase C and ultimately produces an increase in inositol trisphosphate and cytosolic Ca2+. More downstream signaling pathways include activation of PKC and modulation of Ca2+ and K+ ion channels. Activation of signaling pathways independent of G-proteins has also been reported.mGluR5 is predominantly expressed in nervous tissue although expression in several non-neural cell types has also been described. In the brain it is highly expressed in the cortex, basal ganglia and hippocampus.The mGluR5 receptor is involved in several physiological processes such as neuronal development, induction of long-term potentiation (LTP) and depression (LTD) as well as in pathological disorders such as brain trauma, chronic pain, Parkinson's and Huntington's disease.

Alternative names: mGluR5, Metabotropic glutamate receptor 5, GRM5, mGlu5, GPRC1E

Gene ID: 24418

NCBI Accession: NM_000842

UniProt: P31424

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Regulation of long-term Neuronal Synaptic Plasticity