MCU antibody (Mitochondrial Matrix)

Catalog No. ABIN7043332

Product Details anti-MCU Antibody

Target: MCU (Mitochondrial Calcium Uniporter (MCU))

Binding Specificity: AA 191-206, Mitochondrial Matrix

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MCU antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Purpose: A Rabbit Polyclonal Antibody to Mitochondrial Calcium Uniporter

Specificity: N-terminus, mitochondrial matrix

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat,mouse - 15,16 amino acid residues identical

Characteristics: Anti-MCU Antibody (ABIN7043332, ABIN7044117 and ABIN7044118)) is a highly selective antibody directed against an epitope of the human mitochondrial calcium uniporter. The antibody can be used in western blot analysis. It has been designed to recognize MCU from human, mouse, and rat samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: CIEQHQLNKERELIER, corresponding to amino acid residues 191-206 of human mitochondrial calcium uniporter

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: BLP-CC328

Blocking Peptide: BLP-CC328

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for MCU

Target: MCU (Mitochondrial Calcium Uniporter (MCU))

Alternative Name: MCU (MCU Products)

Background: Mitochondrial calcium uniporter, HsMCU, Coiled-coil domain-containing protein 109A, Ccdc109a, C10orf42,Mitochondrial calcium uniporter (MCU) is a 480 kDa mitochondrial inner membrane protein, a multimer that forms a calcium (Ca2+) channel in the lipid bilayer. MCU is one of the most important and highly selective channels responsible for mitochondrial calcium uptake. This uptake plays an important role in cell energy balance and in cell fate determination.The MCU protein is comprised of Mcu which functions as the pore-forming and calcium-conducting subunit, encoded by the Ccdc109a gene. The other units- MICU1 (calcium uptake protein 1, mitochondrial), MICU2 (calcium uptake protein 2, mitochondrial), EMRE (essential MCU regulator, mitochondrial), MCUR1 (mitochondrial calcium uniporter regulator 1) and MCUb (calcium uniporter regulatory subunit MCUb, mitochondrial) all function as regulatory subunits1-3.Studies have demonstrated that knockdown of Mcu uniport in isolated cardiomyocytes enhances peak levels in cytosolic Ca2+ oscillation, resulting in an increase of muscle contraction2.Mcu pore-forming subunit is a homo-oligomer with two coil-coiled domains and two transmembrane domains (TM) separated by a short hydrophilic loop enriched in acidic amino acids. The second transmembrane helices from five Mcu subunits form a hydrophilic pore in the inner membrane of the mitochondria. The DXXE motif forms the pore entrance in the inter-membrane space. The N- and the C-terminus of MCU face the matrix1,3.

Alternative names: MCU, Mitochondrial calcium uniporter, HsMCU, Coiled-coil domain-containing protein 109A, Ccdc109a, C10orf42

Gene ID: 90550

NCBI Accession: NM_138357

UniProt: Q8NE86

Pathways: Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis