NGFR antibody (Intracellular, Juxtamembrane Domain)

Catalog No. ABIN7886733

The Rabbit Polyclonal anti-NGFR antibody is suitable to detect NGFR in samples from Human. It has been validated for WB, IHC and IF.

Quick Overview for NGFR antibody (Intracellular, Juxtamembrane Domain) (ABIN7886733)

Target: NGFR (Nerve Growth Factor Receptor (NGFR))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NGFR antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-NGFR Antibody

Binding Specificity: AA 278-293, Intracellular, Juxtamembrane Domain

Purpose: A Rabbit Polyclonal Antibody to Human Neurotrophin Receptor p75NTR

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,rat - identical

Characteristics: Anti-p75 NGF Receptor (intracellular) Antibody is directed against an epitope of human p75NTR. Anti-p75 NGF Receptor (intracellular) Antibody (ABIN7043348, ABIN7044763 and ABIN7044764) can be used in western blot and immunohistochemistry applications. It has been designed to recognize p75NTR from human, mouse and rat samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: CKQNKQGANSRPVNQT corresponding to amino acid residues 278-293 of human p75NTR 

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:200

IP: The optimal concentration should be determined by the user

Comment:

Cited Application: IP|IHC

Negative Control: (ABIN7236006)

Blocking Peptide: (ABIN7236006)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for NGFR

Target: NGFR (Nerve Growth Factor Receptor (NGFR))

Alternative Name: NGFR

Background:

Synonyms: p75NTR, p75 Neurotrophin receptor, Low affinity NGF receptor, TNFR superfamily member 16, TNFRSF16, CD271

Description: The p75 neurotrophin receptor (p75NTR) is a member of the TNF receptor superfamily. p75NTR, like all the superfamily members is a type I transmembrane protein with tandem cysteine-rich domains in the extracellular portion and a intracellular death domain. p75NTR binds its ligands as a homodimer but can also form heterodimers with other receptors such as TrkA, TrkB, TrkC, Nogo receptor and sortilin. The precise multimeric receptor complex formed between p75NTR and the other receptors will determine the ligand being recognized (see below) and the biological response to its binding.As it name implies, p75NTR binds all the neurotrophins (NGF, BDNF, NT-3 and NT-4) with similar nM affinities. Co-expression of p75NTR with the Trk receptors enhances the ability to bind and respond to the specific neurotrophin and induces cell survival. On the other hand, it has recently been demonstrated that p75NTR binds with high affinity to the unprocessed form of NGF (proNGF) probably as a complex with sortilin and this leads to cell death by apoptosis. Finally, a multimeric complex of p75NTR and Nogo receptor binds myelin proteins such as Nogo-66, MAG and OmgP resulting in inhibition of axonal growth.

Gene ID: 4804

NCBI Accession: NM_002507

UniProt: P08138

Pathways: NF-kappaB Signaling, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis, Growth Factor Binding