NRP2 antibody (Extracellular) (FITC)

Catalog No. ABIN7043381

The Rabbit Polyclonal anti-NRP2 antibody has been validated for FACS. It is suitable to detect NRP2 in samples from Human.

Quick Overview for NRP2 antibody (Extracellular) (FITC) (ABIN7043381)

Target: NRP2 (Neuropilin 2 (NRP2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NRP2 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Product Details anti-NRP2 Antibody (FITC)

Binding Specificity: AA 747-758, Extracellular

Purpose: A Rabbit Polyclonal Antibody to Neuropilin-2 Conjugated to the Fluorescent Dye FITC

Specificity: Extracellular, N-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat,mouse - 11,12 amino acid residues identical

Characteristics: Anti-Neuropilin-2 (NRP2) (extracellular) Antibody (ABIN7043380, ABIN7044700 and ABIN7044701) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunohistochemistry, and indirect flow cytometry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting the protein in living cells. It has been designed to recognize NRP2 from mouse, rat, and human samples. \nAnti-Neuropilin-2 (NRP2) (extracellular)-FITC Antibody (ABIN7043380, ABIN7044700 and ABIN7044701)-F) is directly conjugated to fluorescein isothiocyanate (FITC). This labeled antibody can be used in immunofluorescent applications such as direct live cell flow cytometry.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)REDQGGEWKHGR, corresponding to amino acid residues 747-758 of human neuropilin-2

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: N/A

Comment:

Negative Control: (ABIN7582044)

Blocking Peptide: (ABIN7236118)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for NRP2

Target: NRP2 (Neuropilin 2 (NRP2))

Alternative Name: NRP2

Background: Vascular endothelial cell growth factor 165 receptor 2, VEGF165R2,The neuropilins (NRPs) are single-pass multifunctional transmembrane glycoproteins that play an important role in cell development, immunity and cancer, and physiological and pathological angiogenesis. They were first discovered as regulators of nervous system development, acting as semaphorin co-receptors with plexins, as well as enhancers of cellular responses to members of the vascular endothelial growth factor (VEGF) family.The NRP family comprises two members: Neuropilin-1 (NRP1) and Neuropilin-2 (NRP2), which are usually expressed as homodimers but can also appear as heterodimers. NRP2 contains a large extracellular N-terminal which consists of five domains, a short transmembrane domain and a small cytoplasmic domain1-3. NRP2 is expressed in a wide variety of cells including endothelial cells, neurons, pancreatic islet cells, hepatocytes, melanocytes and osteoblasts. NRP2 has been shown to mediate proliferation, survival, and migration of tumor cells3.NRP2 knockout mice studies show that mice are viable without defects in blood vessels but display abnormal lymphatic development and defects of the central and peripheral nervous systems2.

Alternative names: Neuropilin-2 (NRP2), Vascular endothelial cell growth factor 165 receptor 2, VEGF165R2

Gene ID: 8828

NCBI Accession: NM_003872

UniProt: O60462

Pathways: Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals