Presenilin 2 antibody (Intracellular, N-Term)

Catalog No. ABIN7886515

This Rabbit Polyclonal antibody specifically detects Presenilin 2 in WB and IHC. It exhibits reactivity toward Rat.

Quick Overview for Presenilin 2 antibody (Intracellular, N-Term) (ABIN7886515)

Target: Presenilin 2 (PSEN2) (Presenilin 2 (Alzheimer Disease 4) (PSEN2))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Presenilin 2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Presenilin 2 Antibody

Binding Specificity: AA 33-48, Intracellular, N-Term

Purpose: A Rabbit Polyclonal Antibody to Presenilin-2

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,human - 13,14 amino acid residues identical

Characteristics: Anti-Presenilin-2 Antibody (ABIN7043554, ABIN7044526 and ABIN7044527) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize PSEN2 from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)DSRPGPEDGENTAQWR, corresponding to amino acid residues 33-48 of rat Presenilin-2

Isotype: IgG

Application Details

Application Notes:

WB: 1:200-1:400

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:200

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7236597)

Blocking Peptide: (ABIN7236597)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for Presenilin 2

Target: Presenilin 2 (PSEN2) (Presenilin 2 (Alzheimer Disease 4) (PSEN2))

Alternative Name: PSEN2

Background:

Synonyms: Psen2, Ps2, Psnl2, AD3LP, AD5, E5-1 STM-2

Description: Presenilin 2 (PS2) is the catalytic subunit of a tetrameric complex containing presenilin-1 or 2, anterior pharynx defective 1 (APH1), nicastrin, and PEN-21.The presenilin complex is the founding member of a unique class of GXGD aspartyl proteases that catalyze the cleavage of the transmembrane domains of Type I membrane proteins including amyloid precursor protein (APP) and Notch1. Other members of this protease family include signal peptide peptidases (SPP) and a variety of archaeal homologs2.PS1 and PS2 proteins have nine helical TM domains arranged with the hydrophilic, flexible N terminus in the cytosol and the C terminus protruding into the lumen or extracellular space3.PS2 shares similarity with PS 1 and the crucial difference between them appears to be a "[DIE]xxxL/I/M" motif present only in PS2 which interacts in a phosphorylation dependent manner with AP-1 complexes and targets PS2 to the late endosome/lysosome complex. The absence of this sequence motif in PS1 allows for the wider subcellular location of PS1 in cell membranes4.Presenilin mutations have been shown to be correlated with occurrence of familial Alzheimer's disease5.

Gene ID: 81751

NCBI Accession: NM_000447

UniProt: O88777

Pathways: Notch Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway