SCN3A antibody (3rd Extracellular Loop, Domain 1)

Catalog No. ABIN7887120

This Rabbit Polyclonal antibody specifically detects SCN3A in WB, IF, IC and LCI. It exhibits reactivity toward Rat.

Quick Overview for SCN3A antibody (3rd Extracellular Loop, Domain 1) (ABIN7887120)

Target: SCN3A (Sodium Channel, Voltage-Gated, Type III, alpha Subunit (SCN3A))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SCN3A antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunochromatography (IC), Live Cell Imaging (LCI)

Product Details anti-SCN3A Antibody

Binding Specificity: 3rd Extracellular Loop, AA 277-288, Domain 1

Purpose: A Rabbit Polyclonal Antibody to SCN3A (NaV1.3) Channel

Cross-Reactivity (Details): May recognize NaV1.1 and NaV1.2 due to a moderate homology between the chosen sequence and the sequences of NaV1.1 and NaV1.2 (although there are no five amino acids identical in a row as considered to be the minimum needed for antibody/antigen recognition).

Predicted Reactivity: Mouse,human - 11,12 amino acid residues identical

Characteristics: Anti-SCN3A (Nav1.3) (extracellular) Antibody is directed against an epitope of the rat NaV1.3 channel. Anti-SCN3A (NaV1.3) (extracellular) Antibody (ABIN7043644, ABIN7045250 and ABIN7045251) can be used in western blot, and live cell imaging applications. It has been designed to recognize NaV1.3 from rat, human and mouse samples. The antibody may recognize NaV1.1 and NaV1.2 due to a moderate homology between the chosen sequence and the sequences of NaV1.1 and NaV1.2 (although there are no five amino acids identical in a row as considered to be the minimum needed for antibody/antigen recognition).

Purification: Affinity purified on immobilized antigen.

Immunogen: CSQWPPSDSAFE, corresponding to amino acid residues 277-288 of rat NaV1.3

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Comment:

Cited Application: IHC|FC

Negative Control: (ABIN7236847)

Blocking Peptide: (ABIN7236847)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for SCN3A

Target: SCN3A (Sodium Channel, Voltage-Gated, Type III, alpha Subunit (SCN3A))

Alternative Name: SCN3A

Background:

Synonyms: BIII, Brain type III Na+ channel, Sodium channel protein type 3 subunit alpha

Description: Voltage-gated sodium channels (NaV) are essential for the generation of action potentials and for cell excitability.1 NaV channels are activated in response to depolarization and selectively allow flow of Na+ ions. To date, nine NaV α subunits have been cloned and named NaV1.1-NaV1.9.4-5 The NaV channels are classified into two groups according to their sensitivity to Tetrodotoxin (TTX): TTX-sensitive (NaV1.1, NaV1.2, NaV1.3, NaV1.4, NaV1.6 and NaV1.7) and TTX-resistant (NaV1.5, NaV1.8 and NaV1.9).2-3Mammalian sodium channels are heterotrimers, composed of a central, pore-forming α subunit and two auxiliary β subunits. The expression of the α subunit isoform is developmentally regulated and tissue specific. Sodium channels in the adult central nervous system and heart contain β1 through β4 subunits, whereas sodium channels in adult skeletal muscle have only the β1 subunit.6,7 NaV1.3, also known as SCN3A, is highly expressed in embryonic sensory neurons and CNS, but its level dramatically decreases in adult rodents.8 Up-regulation of NaV1.3 channel expression was described in injured neurons and injured spinal cord.9-11

Gene ID: 497770

NCBI Accession: NM_006922

UniProt: P08104