SCN8A antibody (AA 1042-1061)

Catalog No. ABIN7043651

Product Details anti-SCN8A Antibody

Target: SCN8A (Sodium Channel, Voltage-Gated, Type VIII, alpha (SCN8A))

Binding Specificity: AA 1042-1061

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SCN8A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC)

Purpose: A Rabbit Polyclonal Antibody to NaV1.6 (SCN8A) Channel

Specificity: Intracellular loop between domains II and III

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse - identical, human - 19,20 amino acid residues identical

Characteristics: Anti-NaV1.6 (SCN8A) Antibody (ABIN7043651, ABIN7045231 and ABIN7045232) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize NaV1.6 sodium channel from rat, human, and mouse samples.

Purification: Affinity purified on immobilized antigen.

Grade: KO Validated

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: CIANHTGVDIHRNGDFQKNG, corresponding to amino acid residues 1042-1061 of rat NaV1.6

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IP|IHC|ICC

Negative Control: (ABIN7236866)

Blocking Peptide: (ABIN7236866)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for SCN8A

Target: SCN8A (Sodium Channel, Voltage-Gated, Type VIII, alpha (SCN8A))

Alternative Name: SCN8A (SCN8A Products)

Background: Sodium channel voltage-gated type VIII alpha subunit, NaCh6, PN4, CerIII,Voltage-gated sodium channels (NaV) are essential for the generation of action potentials and for cell excitability.1 NaV channels are activated in response to depolarization and selectively allow flow of Na+ ions. To date, nine NaV α subunits have been cloned and named NaV1.1-NaV1.9.4-5 The NaV channels are classified into two groups according to their sensitivity to tetrodotoxin (TTX): TTX-sensitive (NaV1.1, NaV1.2, NaV1.3, NaV1.4, NaV1.6 and NaV1.7) and TTX-resistant (NaV1.5, NaV1.8 and NaV1.9).2-3Mammalian sodium channels are heterotrimers composed of a central, pore-forming α subunit and two auxiliary β subunits. Expression of the α subunit isoform is developmentally regulated and tissue specific. Sodium channels in the adult central nervous system and heart contain β1 through β4 subunits, whereas sodium channels in adult skeletal muscle have only the β1 subunit.6,7NaV1.6 is highly expressed in the adult brain and localized at high density in Nodes of Ranvier and axon initial segments and at lower density in dendrites and cell bodies of some neurons. NaV1.6 channels are also expressed at high levels in cerebellar Purkinje neurons.8-11

Alternative names: NaV1.6, Sodium channel voltage-gated type VIII alpha subunit, NaCh6, PN4, CerIII

Gene ID: 29710

NCBI Accession: NM_014191

UniProt: O88420

Pathways: Sensory Perception of Sound