TRPM2 antibody (Extracellular)

Catalog No. ABIN7043831

Product Details anti-TRPM2 Antibody

Target: TRPM2 (Transient Receptor Potential Cation Channel, Subfamily M, Member 2 (TRPM2))

Binding Specificity: AA 999-1012, Extracellular

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRPM2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Purpose: A Rabbit Polyclonal Antibody to TRPM2 Channel

Specificity: 3rd extracellular loop

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Human,mouse,rat - 13,14 amino acid residues identical

Characteristics: Anti-TRPM2 (extracellular) Antibody (ABIN7043831, ABIN7044075 and ABIN7044076) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunohistochemistry, and live cell flow cytometry applications. It has been designed to recognize TRPM2 from mouse, rat, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)NGTDPYKPK(S)PESD, corresponding to amino acid residues 999 - 1012 of human TRPM2

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: (ABIN7237447)

Blocking Peptide: (ABIN7237447)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for TRPM2

Target: TRPM2 (Transient Receptor Potential Cation Channel, Subfamily M, Member 2 (TRPM2))

Alternative Name: TRPM2 (TRPM2 Products)

Background: Transient receptor potential cation channel subfamily M member 2, Transient receptor potential melastatin 2, LTrpC2, TrpC7,The mammalian melastatin-related transient receptor potential (TRPM) is a subfamily of the TRP family. The family was named after the first discovered member, melastatin (TRPM1) whose gene was identified in metastatic and benign melanomas1-3. The TRPM family consists of eight members: TRPM1-8 that can be further divided into four pairs: TRPM1 and TRPM3, TRPM2 and TRPM8, TRPM4 and TRPM5, and TRPM6 and TRPM71,2.The TRPM proteins share structural homology with other members of the TRP superfamily channels, six putative transmembrane domains, and cytoplasmic N- and C-termini. However, due to their long N- and C-termini they are also named the long TRP channel family1-3. The C-terminal regions of three TRPM members (TRPM2, TRPM6 and TRPM7) are occupied by enzymatic domains. Almost all the C-terminal region of TRPM2 has an enzymatic domain with sequence similarities to Nudix hydrolases (cleave mononucleotide and dinucleotide polyphosphates2,4.TRPM2 (LTRpC2, TrpC7) is a Ca2+-permeable, nonselective cation channel that is predominantly expressed in various regions of the brain, where it is preferentially localized in microglial cells, the host macrophages of the central nervous system and is also expressed in other tissues, including spleen, heart, liver, lung, and bone marrow. Several splice variants of TRPM2 have been identified5,3.

Alternative names: Transient receptor potential cation channel subfamily M member 2, Transient receptor potential melastatin 2, LTrpC2, TrpC7

Gene ID: 7226

NCBI Accession: NM_003307

UniProt: O94759